Ad hoc Seminar – Francis O’Reilly
“In-cell architecture of coupled transcription and translation”
“Crosslinking mass spectrometry has developed a lot in recent years. I will describe how it is now a robust technique for structural studies of purified complexes, particularly in combination with cryo-electron microscopy (cryo-EM).
Crosslinking mass spectrometry can also be used to study proteins in situ. We have used it in combination with cryo-electron tomography (cryo-ET) to study the physical coupling of the RNA polymerase and ribosome in the simple bacterium Mycoplasma pneumoniae. For the first time, we were able to place the general transcription elongation factors NusG and NusA within this super-complex and suggest how their function could be married with the coupling of transcription and translation.”
Rappsibler Lab, TU Berlin