“The analysis and physical isolation of cells according to their phenotypic and functional properties is an essential technique in cell biology.”

The facility measures the fluorescence emitted by individual cells labelled with fluorescent probes, with specific subpopulations of cells characterised and isolated for further investigation.

A broad spectrum of fluorescent probes are available, including antibodies specific for cell surface or intra-cellular molecules, dyes to determine the cellular content of DNA or other bio-molecules, and indicators of biophysical properties such as membrane potential or intra-cellular second messengers.
The facility provides state of the art instrumentation including two bench-top fluorescence analysers (BD FACSCalibur and BD LSR II) and four fluorescence-activated cell sorters (BD FACSAria IIu, BD FACSAria III, BD Aria Fusion and BD Influx).
Flow Cytometry Facility

BD Aria Fusion – 5 laser sorter capable of sterile 4-way and up to, currently, 384-well plate, sorting – at high precision and purity. Contact us for more details.

Selected Publications

Marcais, A., Blevins, R., Graumann, J., Feytout, A., Dharmalingham, G., Carroll, T., Amado, IF., Bruno, L., Lee, K., Walzer, T., Mann, M., Freitas, AA., Boothby, M., Fisher, AG., Merkenschlager, M. (2014).  microRNA-mediated regulation of mTOR complex components facilitates discrimination between activation and anergy in CD4 T cells. J Exp Med 211 (11): 2281-2295

Tsubouchi, T., Soza-Ried, J., Brown, K., Piccolo, FM., Cantone, I., Landeira, D., Bagci, H., Hochegger, H., Merkenschlager, M., Fisher, AG. (2013). DNA synthesis is required for reprogramming mediated by stem cell fusion. Cell 152:873-83.

Ottone C., Krusche B., Whitby A., Clements M., Quadrato G., Pitulescu M.E., Adams R.H. and Parrinello S. (2014). Direct cell-cell contact with the vascular niche maintains quiescent neural stem cells. Nature Cell Biology, doi:10.1038/ncb3045, published online