“DNA replication and chromosome segregation are two fundamental events during cell reproduction.”
DNA replication and chromosome segregation are two fundamental events during cell reproduction. Eukaryotic genomes are organised into linear molecules of DNA known as chromosomes. Because chromosomal DNA is far too long to fit inside the cell nucleus in its naked form, it is compacted into chromatin fibers. Our laboratory is interested in understanding the molecular mechanisms that regulate genome folding and ensure the faithful segregation of chromosomes during cell division. We study complexes of the SMC (structural maintenance of chromosomes) family; cohesin, condensin, the Smc5/6 complex and their roles in chromosome condensation, sister chromatid cohesion and DNA repair.
The long-term goal in our laboratory is to understand how SMC complexes work at a mechanistic level. We take multidisciplinary approaches toward this goal, including genetics, biochemistry and cell biology.
Comparative kinetics of DNA Double Strand Break repair in cells with (Wt) and without (smc6-9) Smc5/6 function
Esteras, M., Liu, I.C., Snijders, A.P., Jarmuz, A., Aragon, L. (2017). Identification of SUMO conjugation sites in the budding yeast proteome. Microbial Cell 4(10):331-341.
Bermúdez-López M, Villoria MT, Esteras M, Jarmuz A, Torres-Rosell J, Clemente-Blanco A, Aragon L. (2016). Sgs1’s roles in DNA end resection, HJ dissolution, and crossover suppression require a two-step SUMO regulation dependent on Smc5/6. Genes & Development 30(11), 1339-1356.
Sen N, Leonard J, Torres R, Garcia-Luis J, Palou-Marin G, Aragón L. (2016). Physical Proximity of Sister Chromatids Promotes Top2-Dependent Intertwining, Molecular Cell 64(1), 134-147.
Leonard J, Sen N, Torres R, Sutani T, Jarmuz A, Shirahige K, Aragón L. (2015). Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase. Cell Reports 13(11), 2336-2344.
McAleenan A, Clemente-Blanco A, Cordon-Preciado V, Sen N, Esteras M, Jarmuz A, Aragón L. (2013). Post-replicative repair involves separase-dependent removal of the kleisin subunit of cohesin. Nature 493(7431), 250-254.