Chad WhildingMicroscopist | Research Support OfficerDilraj MatharuMicroscopist | Research Support Officer
If you're interested in joining the LMS
We provide the advanced light microscopy equipment, the user training and the image analysis tools required to support LMS research.
The facility offers a large variety of imaging systems, from fluorescence stereo microscopes to the latest super-resolution instruments. Facility staff provide training and support ranging from help with experimental design, image processing/visualisation and quantitative image analysis to direct collaboration on specific projects. This usually involves developing customised software solutions to automate image analysis workflows.
In addition to the cutting edge equipment listed we provide a wide range of fully automated fluorescence imaging workstations for fixed and live cell imaging and a number of basic manually operated microscopes.
Image processing and analysis tasks can be performed on three dedicated high spec image analysis workstations.
Confocal Microscopes :
Lightsheet Imaging:
Super-resolution imaging:
Slide scanner:
MicroCT scanner:
Sun, X. M., Bowman, A., Priestman, M., Bertaux, F., Martinez-Segura, A., Tang, W., Whilding, C., Dormann, D., Shahrezaei, V., Marguerat, S. (2020). Size-Dependent Increase in RNA Polymerase II Initiation Rates Mediates Gene Expression Scaling with Cell Size. Current Biology, 30, no. 7, 1217-1230.
Gutierrez-Escribano P, Newton MD, Llauró A, Huber J, Tanasie L, Davy J, Aly I, Aramayo R, Montoya A, Kramer H, Stigler J, Rueda DS, Aragon L. (2019). A conserved ATP- and Scc2/4-dependent activity for Cohesin in tethering DNA molecules. Sci Adv 5, 6804.
Pollard, A. E., Martins, L., Muckett, P. J., Khadayate, S., Bornot, A., Clausen, M., Admyre, T., Bjursell, M., Fiadeiro, R., Wilson, L., Whilding, C., Kotiadis, V. N., Duchen, M. R., Sutton, D., Penfold, L., Sardini, A., Bohlooly, Y. M., Smith, D. M., Read, J. A., Snowden, M. A., Woods, A., Carling, D. (2019). Ampk Activation Protects against Diet Induced Obesity through Ucp1-Independent Thermogenesis in Subcutaneous White Adipose Tissue. Nature Metabolism, 1, no. 3, 340-349.
Dormann, D. (2019). Intensitycheck – the Light Measuring App for Microscope Performance Checks and Consistent Fluorescence Imaging. PLoS One, 14, no. 3: e0214659.