We develop and apply single-molecule microscopy approaches to image and investigate epigenetic mechanisms involved in human diseases, such as cancer and neurodegenerative disorders, and to interrogate the structural dynamics of protein complexes from the molecule to cellular level.
Alcón, P., Kaczmarczyk, A.P., Ray, K.K. et al. FANCD2–FANCI surveys DNA and recognizes double- to single-stranded junctions. Nature 632, 1165–1173 (2024). https://doi.org/10.1038/s41586-024-07770-w
Kaczmarczyk AP, Déclais AC, Newton MD et al. Search and processing of Holliday junctions within long DNA by junction-resolving enzymes. Nature Communications 13, 5921 (2022). doi: 10.1038/s41467-022-33503-6
Belan O, Barroso C, Kaczmarczyk A, Anand R, Federico S, O’Reilly N, Newton MD, Maeots E, Enchev RI, Martinez-Perez E, Rueda DS, Boulton SJ. (2021). Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins. Molecular Cell 81(5):1058-1073.e7. doi: 10.1016/j.molcel.2020.12.020.
Cawte AD, Unrau PJ and Rueda DS. (2020). Live cell imaging of single RNA molecules with fluorogenic Mango II arrays. Nature Communications 11:1-11
Newton MD, Taylor BJ, Driessen RP, Roos L, Cvetesic N, Allyjaun S, Lenhard B, Cuomo ME & Rueda DS. (2019). DNA stretching induces Cas9 off-target activity. Nature Structural & Molecular Biology, 26, 185-192.
