“We offer a range of services and expertise in the creation, maintenance and study of transgenic mice and rats.”

Established in 1996, the Facility offers the following services:
CRISPR/Cas9 design and in vitro testing; pronuclear injection of DNA and CRISPR; surgical implantation of varying stage embryos; ES cell injection into blastocysts; gene targeting; electroporation; karyotyping; ES cell culture; aggregation; and early embryo manipulation.

In our purpose-built laboratory and CBS suite we also offer a full “cryo” and rederivation service including:
Embryo freezing; sperm freezing; rederivation via IVF/assisted IVF; rederivation via frozen or fresh embryos; arrange imports and exports of sperm/embryos from around the world; and a searchable database of all GM strains across LMS and Imperial sites.

Staff are always on hand to undertake experimental work, advise researchers on experimental design and offer training in the latest techniques available in GM mouse/rat production.

Selected publications

Salker MS, Singh Y, Durairaj RRP, Yan J, Alauddin M, Zeng N, Steel JH, Zhang S, Nautiyal J, Webster Z, Brucker SY, Wallwiener D, Anne Croy B, Brosens JJ, Lang F (2018) LEFTY2 inhibits endometrial receptivity by downregulating Orai1 expression and store-operated Ca2+entry. J Mol Med. 96(2):173-182. doi: 10.1007/s00109-017-1610-9

Coan PM, Barrier M, Alfazema N, Carter RN, Marion de Procé S, Dopico XC, Garcia Diaz A, Thomson A, Jackson-Jones LH, Moyon B, Webster Z, Ross D, Moss J, Arends MJ, Morton NM, Aitman TJ (2017) Complement Factor B Is a Determinant of Both Metabolic and Cardiovascular Features of Metabolic Syndrome. Hypertension 70:624–633 doi: 10.1161/HYPERTENSIONAHA.117.09242.

Van de Pette M, Abbas A, Feytout A, McNamara G, Bruno L, To WK, Dimond A, Sardini A, Webster Z, McGinty J, Paul EJ, Ungless MA, French PMW, Withers DJ, Uren A, Ferguson-Smith AC, Merkenschlager M, John RM, Fisher AG. (2017) Visualizing Changes in Cdkn1c Expression Links Early-Life Adversity to Imprint Mis-regulation in Adults. Cell Rep. 18(5):1090-1099. doi: 10.1016/j.celrep.2017.01.010.

Amouroux R, Nashun B, Shirane K, Nakagawa S, Hill PW, D’Souza Z, Nakayama M, Matsuda M, Turp A, NdjeteheE, Encheva V, Kudo NR, Koseki H, Sasaki H, Hajkova P. (2016) De novo DNA methylation drives 5hmC accumulation in mouse zygotes. Nat Cell Biol. 18(2):225-233. doi: 10.1038/ncb3296.

Nashun B, Hill PW, Smallwood SA, Dharmalingam G, Amouroux R, Clark SJ, Sharma V, Ndjetehe E, Pelczar P, Festenstein RJ, Kelsey G, Hajkova P. (2015) Continuous Histone Replacement by Hira Is Essential for Normal Transcriptional Regulation and De Novo DNA Methylation during Mouse Oogenesis. Mol Cell.19;60(4):611-25. doi: 10.1016/j.molcel.2015.10.010.